Products
Products
Products
| HS Code | 456620 |
| Product Name | Aladdin Isopropyl Alcohol (IPA) - Suitable for Molecular Biology, ≥99.5% (GC) |
| Cas Number | 67-63-0 |
| Molecular Formula | C3H8O |
| Molecular Weight | 60.10 g/mol |
| Purity | ≥99.5% (GC) |
| Grade | Molecular Biology Grade |
| Appearance | Clear colorless liquid |
| Boiling Point | 82.5 °C |
| Melting Point | -89.5 °C |
| Density | 0.785 g/mL at 25 °C |
| Refractive Index | 1.377 at 20 °C |
| Solubility | Miscible in water and most organic solvents |
| Flash Point | 11.7 °C (closed cup) |
| Storage Conditions | Room temperature, tightly closed, in a dry and well-ventilated area |
As an accredited Aladdin Isopropyl Alcohol(IPA) - Suitable for Molecular Biology, ≥99.5%(GC) factory, we enforce strict quality protocols—every batch undergoes rigorous testing to ensure consistent efficacy and safety standards.
| Packing | Packaged in a 500 mL amber glass bottle with secure screw cap, labeled for molecular biology use, ensuring purity and safety. |
| Container Loading (20′ FCL) | One 20-foot container loaded with Aladdin Isopropyl Alcohol (IPA), molecular biology grade, ≥99.5% purity, securely packaged and palletized. |
| Shipping | Aladdin Isopropyl Alcohol (≥99.5%, molecular biology grade) is shipped as a flammable liquid (Class 3) in sealed, UN-approved containers. Transport follows IATA/IMDG/ADR regulations, with proper hazard labeling and documentation. Deliveries exclude air freight unless specially arranged. Handling requires ventilation and avoiding ignition sources. |
| Storage | Store in a tightly sealed, original container in a cool, dry, well-ventilated area away from heat, sparks, and open flames. Keep protected from moisture and contamination to preserve molecular biology grade purity. Avoid contact with strong oxidizing agents. Ensure proper labeling and use appropriate containment to prevent evaporation. |
| Shelf Life | Shelf life is typically 3 years if stored tightly sealed, away from heat, flames, and light, in original container. |
Aladdin Isopropyl Alcohol (IPA) with GC purity ≥99.5% functions in the purification of plasmid DNA from Escherichia coli after alkaline lysis and neutralisation. The cleared lysate is mixed with 0.6–0.7 volumes of IPA per volume of clarified liquid, the exact ratio being shifted toward 0.7 volumes when plasmid copy number is low or when the host strain is HB101 or JM109. Precipitation proceeds for 10 min at ambient temperature, after which a fixed-angle rotor operating at 12,000×g for 15 min collects a glassy translucent pellet in 1.5 mL tubes. For 50 mL high-capacity tubes, the pellet is compacted at 15,000×g for 30 min to prevent floating films. The supernatant is removed, and the pellet is washed with 1 mL of 70% ethanol at -20°C per 1.5 mL tube to strip residual isopropanol and guanidinium salts. Air-drying is limited to 5–10 min because overdried plasmid DNA can lose transform ability and become difficult to resuspend in 10 mM Tris-Cl, 1 mM EDTA, pH 8.0. Terminal product is supercoiled plasmid DNA for restriction digestion, Sanger sequencing, or further purification by anion-exchange chromatography for transfection. Input masses below 5 µg require carrier addition of 10–20 µg glycogen to make pellet recovery reproducible. Lot acceptance for this use is based on the certificate of analysis showing GC purity ≥99.5%, low UV absorbance at 230 nm, 260 nm, and 280 nm, and absence of DNase or nickase activity according to the supplier's molecular-biology release panel.
| Feedstock volume | IPA ratio | Centrifugation | Wash | Terminal form |
|---|---|---|---|---|
| 1.5 mL microcentrifuge tube | 0.6–0.7 vol | 12,000×g, 15 min, 4°C | 1 mL 70% ethanol | plasmid DNA pellet |
| 15 mL conical tube | 0.7 vol | 12,000×g, 30 min, 4°C | 5 mL 70% ethanol | plasmid DNA bulk pellet |
| 50 mL polypropylene tube | 0.7 vol | 15,000×g, 30 min, 4°C | 10 mL 70% ethanol | plasmid DNA bulk pellet |
In phenol–guanidinium isothiocyanate RNA extraction, the upper aqueous phase is mixed with 0.5 volumes of Aladdin IPA per original volume of extraction reagent. For a 1 mL acid-guanidinium phenol-chloroform reaction, this corresponds to 0.5 mL IPA; for a 10 mL scale-up, the addition is 5 mL. The mixture is inverted 10–15 times and incubated at 20–25°C for 10 min. Centrifugation at 12,000×g for 10 min at 4°C produces a pellet that is washed with 1 mL of 75% ethanol per tube and re-centrifuged at 7,500×g for 5 min. Terminal RNA is resuspended in 20–50 µL nuclease-free water or 1 mM sodium citrate pH 6.5 and is used directly for first-strand cDNA synthesis, qPCR, or RNA sequencing library preparation. The operational boundary in this application is that isopropanol precipitation in high-salt guanidinium systems co-precipitates polysaccharides from plant tissues, proteoglycans from animal sources, and high-molecular-weight genomic DNA from bacterial feedstocks; the protocol must therefore include a post-precipitation lithium chloride wash for polysaccharide removal when the source is plant leaf material. High-purity molecular-biology IPA reduces metal-cation-induced RNA hydrolysis during overnight storage at -80°C and maintains the RNA integrity number above 8.0 when measured by capillary electrophoresis. Residual chloride or acetate salts from the precipitation step can inhibit downstream reverse transcription if the pellet is not completely drained, so wash efficiency is monitored by resuspension conductivity below 10 µS/cm.
Aladdin IPA serves as the organic eluent in ion-pair reversed-phase high-performance liquid chromatography of synthetic oligonucleotides, where the substitution of acetonitrile by IPA changes retention selectivity for phosphorothioate or 2'-O-methyl modifications. The mobile phase A is 100 mM triethylammonium acetate at pH 7.0; mobile phase B is prepared as 50–90% IPA in 100 mM triethylammonium acetate. A typical gradient elevates IPA from 5% to 25% over 20 min at 1.0 mL/min through a 4.6 × 150 mm, 5 µm C18 column maintained at 60°C. The higher column temperature is necessary because IPA has a dynamic viscosity near 2.04 cP at 25°C, and the resulting backpressure can be 30–40% higher than an equivalent acetonitrile method. The terminal product is a peak-fractionated, detritylated oligonucleotide of 95–99% full-length purity after vacuum lyophilisation, used for antisense testing, probe synthesis, or sequencing primer production. System suitability is evaluated according to USP <621> criteria for tailing factor and plate count before each batch, and the UV detector is set at 260 nm with a baseline drift specification below 0.05 AU during blank gradients. The use of this molecular-biology grade avoids the broad solvent front at 230 nm that is a known limitation of lower-purity technical IPA. Because IPA is more hydrophobic and less polar than acetonitrile, gradient dwell volume and column regeneration with 100% IPA must be revalidated before adoption; otherwise carryover of early-eluting failure sequences can appear in the next chromatographic run.
Seventy percent IPA is prepared by combining 70 mL Aladdin IPA with 30 mL nuclease-free water in a sterile polypropylene vessel, not borosilicate glassware, to limit trace-metal leaching. The mixture is applied to automated liquid-handler decks, benchtop work surfaces, pipette shafts, and centrifuge rotor exteriors using hydroentangled polyester wipes that have been pre-washed to remove detergent residues. Contact time is held at 1–2 min for general surface load reduction, after which surfaces are allowed to air-dry before UV irradiation. The terminal product is a PCR setup environment with reduced carryover of dried nucleotide solutions, polymerase inhibitors, and salt films that otherwise interfere with high-fidelity amplification. This application has an operational boundary: 70% IPA does not inactivate RNase A, and therefore RNA-extraction and RNA-work areas must be treated separately with DEPC-treated water or a commercial RNase-inactivating solution. The diluted IPA should not be applied directly to open pipette tip racks, PCR plates, or consumable interiors because the solvent can mobilise plastic additives and leave non-volatile films. For cleanroom operation, the wiping procedure is performed under the facility SOP aligned with ISO 14644-1:2015 Class 7 conditions, and the wiped surface is validated by ATP bioluminescence or contact-plate microbial counts.
After ammonia cleavage of synthetic oligodeoxynucleotides from controlled-pore-glass support, the ammoniacal cleavage solution is evaporated to dryness in a vacuum concentrator at 55°C. The dried residue is resuspended in 0.3 M sodium acetate pH 5.2, and 0.7 volumes of Aladdin IPA are added. The mixture is incubated at -20°C for 1 h and then centrifuged at 13,000×g for 30 min at 4°C. The resulting pellet is washed with 1 mL cold 70% ethanol and re-centrifuged at 13,000×g for 10 min. The terminal product is a desalted oligonucleotide powder for PCR primers, sequencing primers, or probes, with residual ammonia and protecting-group fragments reduced to levels compatible with carbodiimide-mediated conjugation. This precipitation step is particularly relevant when downstream ligation or amine-reactive labelling would be inhibited by the high salt concentration of crude cleavage mixtures. Because the separation discriminates by length-dependent solubility, the water content of IPA must be controlled: excessive water increases the solubility of truncated failure sequences and reduces the removal of short-mer impurities. The pellet is briefly air-dried for 3–5 min to avoid incomplete resuspension, then reconstituted in 10 mM Tris-HCl pH 8.0.
Aladdin IPA is used as a volatile precipitant in hanging-drop and sitting-drop vapor-diffusion experiments at working concentrations between 10% and 40% v/v. A representative reservoir condition is 20–25% IPA, 0.1 M sodium citrate pH 5.6, and 0.2 M ammonium acetate; protein solution at 5–10 mg/mL is mixed 1:1 with the reservoir and equilibrated against the reservoir at 18°C. Drops of 1–2 µL are dispensed on siliconised cover slides and sealed over the reservoir with vacuum grease. The terminal product is diffraction-quality single crystals for X-ray data collection at synchrotron or rotating-anode beamlines. Because IPA can slowly oxidise to acetone and aldehydes, the use of a high-purity molecular-biology lot limits the concentration of protein-modifying contaminants that would otherwise alter surface lysine residues and shift the crystallisation drop pH. The solution is filtered through a 0.22 µm polyethersulfone filter before use to remove insoluble particulate matter that can act as secondary nucleation centres. Published data for this specific configuration is limited because crystallisation outcomes are strongly protein-dependent; IPA is less commonly used as a primary precipitant than polyethylene glycol or ammonium sulfate, and its success is generally restricted to proteins with lower surface hydrophobicity.
Competitive Aladdin Isopropyl Alcohol(IPA) - Suitable for Molecular Biology, ≥99.5%(GC) prices that fit your budget—flexible terms and customized quotes for every order.
For samples, pricing, or more information, please contact us at +8618136850665 or mail to sales4@ascent-chem.com.
We will respond to you as soon as possible.
Tel: +8618136850665
Email: sales4@ascent-chem.com
Flexible payment, competitive price, premium service - Inquire now!
Aladdin Isopropyl Alcohol(IPA) - Suitable for Molecular Biology, ≥99.5%(GC) is a single-component solvent identified by CAS 67-63-0, linear formula C3H8O, and molar mass 60.10 g/mol. At 101.3 kPa, the boiling point is 82.6 °C; the density at 20 °C is 0.786 g/cm³ and the closed-cup flash point is approximately 12 °C. The model designation is a molecular-biology-grade isopropanol with area-normalized GC-FID assay ≥99.5%; water is not included in that assay and is specified separately by ASTM E203 Karl Fischer titration. The product is supplied without intentional addition of denaturants, detergents, or bittering agents. In laboratory use, it functions as an anhydrous precipitation solvent for nucleic acids, a wash solvent for extracted DNA and RNA, and a low-residue cleaning solvent for equipment surfaces where UV-absorbing and non-volatile contamination must be controlled.
The ≥99.5% (GC) descriptor should not be read as total purity. Gas chromatography measures volatile organic impurities and solvent area percentage; it does not measure water, non-volatile residue, or particulate matter. Molecular biology workflows therefore require lot-specific confirmation of water, evaporation residue, UV absorbance, and redox-active impurities. The product differs from general industrial isopropanol primarily in the control of these non-GC impurities, while the base solvent identity remains the same.
In DNA precipitation, the addition of 0.6–1.0 volume of isopropanol to an aqueous DNA solution containing 0.3 M sodium acetate at pH 5.2 or 0.8 M sodium chloride lowers the dielectric constant of the medium and drives hydrated nucleic acid into an insoluble aggregate. The final isopropanol concentration in a typical 0.7-volume addition is approximately 41% v/v when the reagent is dry; a water content of ≤0.1% shifts this value by less than 0.1 percentage point. Industrial-grade IPA with 0.5–1.0% water requires volume compensation and introduces batch-to-batch variability in small-scale extractions. Recovery is performed in fixed-angle rotors, commonly at 20,000×g for 20–30 min at 4 °C. The precipitated nucleic acid is washed with 75% ethanol to remove residual isopropanol and salt; failure to remove solvent can inhibit Taq DNA polymerase and reverse transcriptase reactions. The low aldehyde and peroxide content of molecular-biology-grade isopropanol is relevant because aldehydes can form Schiff bases with exocyclic amino groups on guanine and cytosine, and peroxides can oxidize 2'-deoxyribose residues under aerobic storage. These contaminants are not reflected in a GC assay if they are non-volatile or co-elute with the solvent; separate lot-specific tests are therefore necessary.
Non-volatile residue is measured by evaporating a known mass in a heated vessel and weighing the remaining material under a procedure such as ASTM D1353. For molecular-biology-grade IPA, routine acceptance limits are established at ≤0.0005% residue after evaporation, ≤0.1% water by ASTM E203, and APHA color not greater than 10 by ASTM D1209. UV absorbance is measured in a 1 cm quartz cell against water, with typical limits of ≤0.05 AU at 260 nm and ≤0.03 AU at 280 nm. These thresholds are operationally important when the solvent is used for precipitating or washing nucleic acids later quantified by UV spectrophotometry. Residual UV-absorbing species can falsely elevate A260 readings and distort the A260/A280 ratio used to estimate DNA purity. Peroxide content, generally controlled at ≤5 ppm as hydrogen peroxide, and aldehyde/ketone content, generally controlled at ≤10 ppm, reduce the risk of adduct formation and oxidative damage during storage of precipitated nucleic acids. The specification “suitable for molecular biology” does not automatically include a DNase/RNase certificate because the solvent is nearly anhydrous and nucleases require an aqueous environment; however, post-opening ingress of aqueous aerosols must be prevented to maintain this operational boundary.
| Parameter | Test method | Typical acceptance criterion |
|---|---|---|
| Assay | GC-FID area normalization | ≥99.5% |
| Water | ASTM E203 Karl Fischer | ≤0.1% |
| Non-volatile residue | ASTM D1353 | ≤0.0005% |
| UV absorbance at 260 nm | 1 cm quartz cell, water blank | ≤0.05 AU |
| UV absorbance at 280 nm | 1 cm quartz cell, water blank | ≤0.03 AU |
| Peroxide as H2O2 | iodometric titration | ≤5 ppm |
| Aldehydes/ketones | DNPH derivatization/HPLC | ≤10 ppm |
| Color | ASTM D1209 | ≤10 APHA |
On automated liquid-handling platforms such as Tecan Freedom EVO and Hamilton Microlab STAR, low non-volatile residue is a direct operational boundary. A residue limit of 0.0005% corresponds to approximately 4 mg of non-volatile material per liter of isopropanol at 20 °C, whereas a general-purpose solvent with 0.005% residue may introduce approximately 39 mg per liter. Repeated aspiration and dispensing through PTFE rotary valves and stainless-steel syringe bores can concentrate this material as a film; the resulting dispense-volume drift is a recognized failure mode when industrial-grade alcohol solvents are substituted in automated nucleic acid extraction. The product is used as a system-fluid wash and as a precipitation solvent in 96-well and 384-well process formats. In such applications, pipette tip residue can be assessed gravimetrically after evaporation of 10 µL aliquots on an analytical balance, with acceptance thresholds tied to instrument service intervals. The absence of added detergents is relevant because non-ionic and ionic surfactants in low-grade alcohol produce stable foam in vacuum-assisted elution plates, interfering with drainage and causing well-to-well cross-talk. Published data for this specific configuration is limited; qualification therefore relies on lot-specific certificate-of-analysis values and in-house residue monitoring.
Capillary sequencing and next-generation library preparation steps use isopropanol for desalting and size-selection washes. The low water content reduces bead clumping in solid-phase reversible immobilization workflows, where stock isopropanol is mixed with polyethylene glycol and sodium chloride to prepare 70% v/v working solutions. In such protocols, the critical parameter is the absence of non-volatile residue after washing, because residual material can suppress cluster amplification on Illumina flow cells. This product is not certified as nuclease-free, so aqueous buffers prepared from it should be filtered through 0.22 µm PVDF membranes where downstream amplification requires sterility. Compared with HPLC-grade isopropanol, the molecular-biology grade may be less restrictive at very short UV wavelengths but places greater emphasis on aldehyde, ketone, and peroxide control relevant to nucleic acid integrity.
The replacement is not interchangeable. Aqueous 70% isopropanol is a surface-disinfectant solution that contains approximately 30% water and is not suitable for selective nucleic acid precipitation. In a monophasic phenol/guanidinium isothiocyanate extraction, aqueous-phase RNA is precipitated with 1 volume of anhydrous ≥99.5% IPA after phase separation. The mixture is incubated at 15–25 °C for 10 min or at −20 °C for 30–60 min, then centrifuged at 12,000×g for 15 min at 4 °C. The pellet is washed with 75% ethanol and air-dried for 5–10 min. If 70% isopropanol is substituted in this step, the increased water content changes the dielectric environment and reduces high-molecular-weight RNA recovery; the resulting pellet is often loose and difficult to visualize. Conversely, the ≥99.5% product should not be diluted to 70% and used as a validated disinfectant unless the final solution meets local biocidal-product regulations and is tested under EN 1276 or ASTM E1053 with the intended organism and contact time. Those disinfectant efficacy standards are outside the molecular biology application of the anhydrous solvent.
| Property | Molecular-biology IPA ≥99.5% (GC) | General industrial IPA | Aqueous 70% isopropanol |
|---|---|---|---|
| Water content | ≤0.1% | 0.5–2.0% | 30% v/v |
| Non-volatile residue | ≤0.0005% | often ≤0.005% or unspecified | not specified |
| UV absorbance at 260 nm | ≤0.05 AU | not specified | not applicable |
| Intentional denaturant or additive | absent | may contain process stabilizers | water-diluted disinfectant |
| Primary laboratory use | nucleic acid precipitation, pellet wash, instrument cleaning | industrial thinning, surface preparation | hard-surface disinfection per validated contact time |
Lot traceability is maintained through the supplier certificate of analysis, which should be retained for the duration of the project. In regulated laboratories, acceptance of this solvent for use in ISO/IEC 17025 workflows requires verification against an internal specification before first use. Important checks include appearance, water content by Karl Fischer, evaporation residue, and UV absorbance at 260 nm. Users should not extrapolate a single lot result to all future lots; analytical-grade substitutes should be re-qualified under the same criteria because carbon feedstocks and purification trains vary among production sites. Where a certificate of analysis is unavailable for a given lot, the solvent should be treated as laboratory-grade until the critical molecular-biology parameters are reconfirmed by the end-user.
Store the solvent in the original tightly closed glass or HDPE container at 15–25 °C, protected from direct sunlight and separated from strong oxidizers, acid chlorides, and acid anhydrides. Isopropanol is classified as flammable under CLP H225 with a closed-cup flash point of 12 °C, autoignition temperature of 399 °C, lower explosive limit of 2.0% v/v, and upper explosive limit of 12.7% v/v. Secondary alcohols form low levels of peroxides on prolonged light and air exposure; the product is not a high-risk peroxide former such as diethyl ether or tetrahydrofuran, but headspace ingress should be minimized after opening. Peroxide test strips are used before any distillation or evaporation step; if peroxides exceed 5 ppm, the solvent should not be concentrated by distillation. The solvent is incompatible with strong oxidizing agents, including perchloric acid and chromium trioxide, and with aluminum under conditions that expose fresh metal surfaces at elevated temperature. Operational boundaries include storage below 30 °C, use inside a fume hood for open transfers above 100 mL, and grounding of stainless-steel containers during liquid transfer to avoid static discharge. The product is not intended for direct therapeutic or pharmaceutical excipient use and is not certified as a sterilant or medical-device disinfectant; its molecular biology use is limited to nucleic acid precipitation, washing, and low-residue laboratory cleaning.
